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Solid State Lasers and Laser Diodes from RPMC Lasers Inc » Products » Fluorescence Lifetime Lasers

Fluorescence Lifetime Lasers

What is Fluorescence Lifetime?

Fluorescence lifetime is a spectroscopy method used for measuring the time a molecule spends in the excited state, by calculating the time between when the laser is fired, and the fluorescence signal is detected as well as the rise and decay time of the signal.  Typical lifetimes are on the order of a few picoseconds to hundreds of nanoseconds depending on the molecule, therefore ultra-short pulsed in the picosecond or femtosecond range are required from fluorescence lifetime lasers. Fluorescence lifetime is an intrinsic property, which does not depend on molecular concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity.  The lifetime is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers.  This makes it an ideal method for differentiating between molecules in messy biological systems, for example, fluorescence lifetime lasers have been shown to distinguish between healthy and cancers brain tissue in real-time.  On this page, you will find a list of all of the full range of fluorescence lifetime lasers we offer at RPMC, including picosecond pulsed visible lasers and femtosecond mode-locked lasers which can be used to induce two-photon fluorescence.

Lasers and Fluorescence Lifetime

3 Important Things When Choosing a Laser Combiner for Flow Cytometry

3. Modulation: The final primary consideration when choosing a laser combiner for flow cytometry are the modulation and triggering capabilities. Each laser must be individually addressable to trigger each of them independently or all at once, ensuring that the wavelengths of interest are incident on the cell stream at the appropriate time.   Traditionally lasers for flow cytometry have been used in a CW or quasi-CW fashion, but recently there has been increased interest in measuring fluorescence lifetime to gain even more information.  In these cases, a pulse width of a few nanoseconds is needed.  The LaserBoxx is capable of providing both of these functionalities, by either direct modulation of the diode lasers, or by adding an Acousto-Optic Modulator (AOM) in between a DPSS laser and the dichroic mirror.

Read the full article here.

How Can We Help?

With many years experience providing fluorescence lifetime lasers to researchers 100s of units fielded, we have the experience to ensure you get the right product for the application. Working with RPMC ensures you are getting trusted advice from our knowledgeable and technical staff on a wide range of laser products.  RPMC and our manufacturers are willing and able to provide custom solutions for your unique application.

If you have any questions, or if you would like some assistance please Contact Us here. Furthermore, you can email us at info@rpmclasers.com to talk to a knowledgeable Product Manager.

Alternatively, use the filters on this page to assist in narrowing down the selection of fluorescence lifetime lasers for sale. Finally, head to our Knowledge Center with our Lasers 101 page and Blogs, Whitepapers, and FAQ pages for further, in-depth reading.

Finally, check out our Limited Supply – In Stock – Buy Now page: This page contains an ever-changing assortment of various types of new lasers at marked-down/discount prices.

 

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Picture Part Number Type Output power (W) Wavelength (nm) Output Linewidth Pulse energy (uJ) Pulse width Rep rate
GBS-C: Broadband Light Source 1.5um Broadband CW Fiber Lasers CW Fiber Lasers 0.160 1529-1610 SM Fiber, PM Fiber Broadband
cpfl 1.5a 1.5at 1.5t cpfl2 hfl 1.5um Pulsed Fiber Lasers Pulsed Fiber Lasers 0.150, 1.0, 5.0 1540-1560 5.0, 25.0, 80.0 400ps, 50ns 5kHz, 1MHz, 2MHz
Pulsed Fiber Laser 1um Pulsed Fiber Lasers Pulsed Fiber Lasers 5.0 1030-1100 150.0 400ps, 50ns 10kHz, 100kHz, 1MHz
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 10XX Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 2.0, 5.0 1040, 1064 0.025, 0.062 100fs, 110fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 520 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 0.800, 2.0 520 0.025 180fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 780 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 0.800 780 0.010 150fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 920 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 1.5, 2.5, 4.0 920 0.0187, 0.0312, 0.050 100fs, 110fs, 130fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR Dual Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 1.0, 2.0 920/1064 0.0125, 0.025 100fs, 110fs, 130fs 80MHz (Optional: 40MHz)
Altair: Femtosecond Laser Altair Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 10.0, 20.0 1040 0.125, 0.250, 1.0 150fs, 250fs 30MHz, 80MHz, Single shot to 40MHz
Antares Antares Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 10.0, 20.0, 30.0 1030, 1064 0.125, 0.250, 0.375, 0.500 10ps 80MHz
DIADEM Series Diadem 1300 Pulsed DPSS Lasers, Ultrafast Lasers 2.0 1300 1.0 150fs Single shot to 2MHz
DIADEM Series Diadem IR Pulsed DPSS Lasers, Ultrafast Lasers 10.0, 20.0, 30.0 1030, 1064 10.0, 20.0, 30.0, 40.0 400fs, 10ps Single shot to 2MHz
Bright Microlaser Microchip SB1 Laser SB1-1064 Pulsed DPSS Lasers, Microchip Lasers 0.008, 0.012, 0.040, 0.110, 0.150, 0.200, 0.225, 0.400 1064 2.0, 15.0, 20.0, 30.0, 40.0, 60.0, 80.0 400ps, 1.3ns 100Hz, 200Hz, 1kHz, 5kHz, 10kHz, 15kHz, 55kHz, 100kHz
Bright Microlaser Microchip SB1 Laser SB1-266 Pulsed DPSS Lasers, Microchip Lasers 0.002, 0.005 266 0.500, 1.0, 2.0 1.3ns 1kHz, 5kHz, 10kHz
Bright Microlaser Microchip SB1 Laser SB1-355 Pulsed DPSS Lasers, Microchip Lasers 0.010 355 Narrow 1.0 1.3ns 5kHz, 10kHz
Bright Microlaser Microchip SB1 Laser SB1-532 Pulsed DPSS Lasers, Microchip Lasers 0.004, 0.006, 0.010, 0.017, 0.020, 0.030, 0.040, 0.075, 0.100 532 0.300, 7.0, 10.0, 15.0, 20.0, 30.0, 40.0 400ps, 1.3ns 10Hz, 100Hz, 200Hz, 1kHz, 5kHz, 10kHz, 15kHz, 55kHz, 100kHz
Bright Microlaser Microchip SB1 Laser SB1-946 Pulsed DPSS Lasers, Microchip Lasers 0.004, 0.060, 0.070, 0.105 946 7.0 2ns 100Hz, 5kHz, 10kHz
Sirius-532: Picosecond DPSS lasers Sirius GR Pulsed DPSS Lasers, Ultrafast Lasers 2.0 532 32.0 10ps Single shot to 1MHz
Sirius-532: Picosecond DPSS lasers Sirius IR Pulsed DPSS Lasers, Ultrafast Lasers 5.0 1064 60.0 10ps Single shot to 1MHz
Wedge-B-1064 Wedge-HB/XB 1064 Pulsed DPSS Lasers 4.0 1064 2000.0, 4000.0 1.5ns Single shot to 1kHz, Single shot to 2kHz
Wedge-B-532 Wedge-HB/XB 532 Pulsed DPSS Lasers 2.0 532 1000.0, 2000.0 1.5ns Single shot to 1kHz, Single shot to 2kHz
Wedge-F-1064 Wedge-HF/XF 1064 Pulsed DPSS Lasers 1.5, 4.0 1064 70.0, 180.0 400ps - 1.6ns, 700ps - 2.5ns 10kHz to 100kHz, 50kHz to 200kHz
Wedge-F-266 Wedge-HF/XF 266 Pulsed DPSS Lasers 0.050, 0.180 266 5.0, 15.0 400ps - 700ps, 500ps - 1ns 10kHz to 50kHz
Wedge-HF-355: 355nm compact nanosecond laser Wedge-HF/XF 355 Pulsed DPSS Lasers 0.800 355 40.0 500ps - 1ns 10kHz to 50kHz
Wedge-F-532 Wedge-HF/XF 532 Pulsed DPSS Lasers 0.500, 1.5, 2.0, 4.0 532 30.0, 40.0, 80.0, 120.0 400ps - 1ns, 500ps - 1.5ns, 750ps - 2ns, 750ps - 2.5ns 10kHz to 100kHz, Single shot to 10kHz
Wedge-XB 1572 Pulsed DPSS Lasers 0.800 1572 8000.0 2.5ns 1kHz
Wedge-XB 3um Pulsed DPSS Lasers 0.200 3106 100.0 3ns Single shot to 2kHz
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FL CW/CW Modulated Series

FL CW/CW Modulated SeriesRPMC offers a variety of standard and custom CW fiber laser options. Available in both OEM and Turnkey formats, our 1um, 1.5um and 2um fiber lasers are manufactured to Telcordia standards and can be modified to meet your applications requirements. With available powers up to 100W at 1um, 30W at 1.5um and 40W at 2um, our CW fiber lasers are suitable for a wide range of applications. Available options and configurations include narrow linewidth, single frequency outputs, C and L-band broadband sources, polarize maintaining fiber options, power tunability and high-speed triggering and modulation. Customized configurations are available upon request.

FL-P Series

1um 1.5um Pulsed Fiber LasersRPMC offers a variety of standard and custom pulsed fiber laser options. Available in both OEM and Turnkey formats, our 1um, 1.5um and 2um fiber lasers are manufactured to Telcordia standards and can be modified to meet your applications requirements. With available average powers up to 10W at 1um and 1.5um, peak powers up to 25kW, pulse widths in the range of 200ps to 100ns, and available options such as pulse monitoring, internal and external triggering, TTL or LVDS input signals, extended operating temperature range, and a robust design, our pulse series of fiber lasers are suitable for a wide range of applications. Customized configurations are available upon request.

Alcor Series

Alcor

ALCOR is the most advanced femtosecond fiber laser emitting at 920 nm or 1064 nm, designed explicitly for multiphoton microscopy instrumentation and industrial OEM integration, working with 24/7 operation in an ultra-compact, robust and air-cooled format. Featuring the Alcor 920 4W, with the highest average power commercially available, as well as the XSight and FLeX Fiber external modules, allowing for fine & fast gating and power control, GDD precompensation, and fiber-coupling (FleX Fiber). 520 nm, 780 nm, and 1040 nm versions also available.

Altair Series

Altair: Femtosecond Laser

The ALTAIR is an ideal solution for biophotonics applications such as multiphoton microscopy where deep excitation of red-shifted indicators such as RCaMP, dtTomato, and MCherry is required.  Providing <160fs pulses, average power up to 20W and peak power up to 1.5MW at 1µm, the ALTAIR is the preferred solution for both OEM and researchers in the microscopy and lifesciences fields.  The fiber-based design ensures robust and reliable 24/7 operation while providing supreme pulse quality from a user friendly, ultra compact air-cooled package.

Antares Series

Antares

ANTARES is a picosecond, quasi-continuous wave laser with a high repetition rate up to 80 MHz and a narrow linewidth, in a very compact and robust format. This laser series offers average powers of 1 to 40W at 1030 nm or 1064 nm (upon request) and can be doubled or tripled in the harmonics to answer even the most challenging industrial and scientific applications. ANTARES is ideally suited for applications such as CARS and spectroscopy applications.

Diadem Series

DIADEM Series

DIADEM Series is a compact, air-cooled, high energy ultrafast laser for advanced micro-machining applications that require short femtosecond pulse widths. The Diadem is available with up to 40µJ of pulse energy, 30W of average power, and operation up to 1MHz (standard), with pulses below <400 fs and an M² < 1.2. This laser has been specifically designed for demanding medical implantable device and electronics manufacturing as well as high peak power optogenetics applications.

Microchip Series

Bright Microlaser Microchip SB1 Laser

The Microchip Series, from BrightMicroLaser, are ultra-compact, passively q-switched, single longitudinal mode (SLM), narrow linewidth, DPSS lasers that feature pulse durations from 400 ps to 2 ns, energy levels up to 80 uJ and repetition rates up to 100 kHz. In addition to the fundamental 1064 nm wavelength and its harmonics (532, 355 and 266 nm), we also deliver units emitting at 946 and 473 nm.

Sirius Series

Sirius-532: Picosecond DPSS lasers

SIRIUS is a very compact, high energy hybrid ultrafast laser for advanced micro-machining applications and supercontinuum pumping, offering over 120µJ and > 5 W, with pulses below 10 ps from single shot to 1MHz and an M² of < 1.2. This laser series specifically designed to allow for ergonomic and user-friendly operation. SIRIUS is ideally suited for selective ablation, electronics, and photonic applications.

Wedge Series

Wedge-XF-1064: 1064nm Picosecond Laser

The Wedge series of DPSS Lasers have been designed for OEM applications like micro machining of hard and soft materials, specialty marking, glass and crystals engraving, LIDAR, LIBS, Spectroscopy and medical diagnostics. High peak powers, with relatively low energy and heat generation, allows efficient ablation and non-linear interaction with most materials. The compact and lightweight package represents a great benefit in LIDAR and other aerospace applications, while short pulses give extremely precise time-of-flight measurements.
Second Harmonic (532 nm output) and wavelength conversion option widen the product application range.