SHIPS TODAY
0
Cart
Solid State Lasers and Laser Diodes from RPMC Lasers Inc » Products » Fluorescence Lifetime Lasers

Fluorescence Lifetime Lasers

What is Fluorescence Lifetime?

Fluorescence lifetime is a spectroscopy method used for measuring the time a molecule spends in the excited state, by calculating the time between when the laser is fired, and the fluorescence signal is detected as well as the rise and decay time of the signal.  Typical lifetimes are on the order of a few picoseconds to hundreds of nanoseconds depending on the molecule, therefore ultra-short pulsed in the picosecond or femtosecond range are required from fluorescence lifetime lasers. Fluorescence lifetime is an intrinsic property, which does not depend on molecular concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity.  The lifetime is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers.  This makes it an ideal method for differentiating between molecules in messy biological systems, for example, fluorescence lifetime lasers have been shown to distinguish between healthy and cancers brain tissue in real-time.  On this page, you will find a list of all of the full range of fluorescence lifetime lasers we offer at RPMC, including picosecond pulsed visible lasers and femtosecond mode-locked lasers which can be used to induce two-photon fluorescence.

Lasers and Fluorescence Lifetime

3 Important Things When Choosing a Laser Combiner for Flow Cytometry

3. Modulation: The final primary consideration when choosing a laser combiner for flow cytometry are the modulation and triggering capabilities. Each laser must be individually addressable to trigger each of them independently or all at once, ensuring that the wavelengths of interest are incident on the cell stream at the appropriate time.   Traditionally lasers for flow cytometry have been used in a CW or quasi-CW fashion, but recently there has been increased interest in measuring fluorescence lifetime to gain even more information.  In these cases, a pulse width of a few nanoseconds is needed.  The LaserBoxx is capable of providing both of these functionalities, by either direct modulation of the diode lasers, or by adding an Acousto-Optic Modulator (AOM) in between a DPSS laser and the dichroic mirror.

Read the full article here.

How Can We Help?

With many years experience providing fluorescence lifetime lasers to researchers 100s of units fielded, we have the experience to ensure you get the right product for the application. Working with RPMC ensures you are getting trusted advice from our knowledgeable and technical staff on a wide range of laser products.  RPMC and our manufacturers are willing and able to provide custom solutions for your unique application.

If you have any questions, or if you would like some assistance please Contact Us here. Furthermore, you can email us at info@rpmclasers.com to talk to a knowledgeable Product Manager.

Alternatively, use the filters on this page to assist in narrowing down the selection of fluorescence lifetime lasers for sale. Finally, head to our Knowledge Center with our Lasers 101 page and Blogs, Whitepapers, and FAQ pages for further, in-depth reading.

Finally, check out our Limited Supply – In Stock – Buy Now page: This page contains an ever-changing assortment of various types of new lasers at marked-down/discount prices.

 

Additional Resources

Whitepapers:

Blogs:

Videos:


Show more
Show less
Filters Reset

Type

Wavelength Selection

Power Selection

Energy Selection

Pulse Width Selection

Rep Rate Selection

Output

Linewidth

Series

more more
Picture Part Number Type Output power (W) Wavelength (nm) Output Linewidth Pulse energy (uJ) Pulse width Rep rate
GBS-C: Broadband Light Source 1.5um Broadband CW Fiber Lasers CW Fiber Lasers 0.160 1529-1610 SM Fiber, PM Fiber Broadband
cpfl 1.5a 1.5at 1.5t cpfl2 hfl 1.5um Pulsed Fiber Lasers Pulsed Fiber Lasers 0.150, 1.0, 4.0 1530-1564 5.0, 25.0, 80.0 400ps, 50ns 5kHz, 1MHz, 2MHz
Pulsed Fiber Laser 1um Pulsed Fiber Lasers Pulsed Fiber Lasers 5.0 1030-1100 150.0 400ps, 50ns 10kHz, 100kHz, 1MHz
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 10XX Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 2.0, 5.0 1040, 1064 0.025, 0.062 100fs, 110fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 520 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 0.800, 2.0 520 0.025 180fs 80MHz (Optional: 40MHz)
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 780 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 0.800 780 0.010 150fs 80MHz
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR 920 Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 1.5, 2.5, 4.0 920 0.0187, 0.0312, 0.050 100fs, 110fs, 130fs 80MHz
ALCOR 5W: 1064nm Ultra-Compact Femtosecond Laser ALCOR Dual Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 1.0, 2.0 920/1064 0.0125, 0.025 100fs, 110fs, 130fs 80MHz
Altair: Femtosecond Laser Altair Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 10.0, 20.0 1040 0.125, 0.250, 1.0 150fs, 250fs 30MHz, 80MHz, Single shot to 40MHz
Antares Antares Pulsed DPSS Lasers, Pulsed Fiber Lasers, Ultrafast Lasers 10.0, 20.0, 30.0 1030, 1064 0.125, 0.250, 0.375, 0.500 10ps 80MHz
DIADEM Series Diadem 1300 Pulsed DPSS Lasers, Ultrafast Lasers 2.0 1300 1.0 150fs Single shot to 2MHz
DIADEM Series Diadem IR Pulsed DPSS Lasers, Ultrafast Lasers 10.0, 20.0, 30.0 1030, 1064 10.0, 20.0, 30.0, 40.0 400fs, 10ps Single shot to 2MHz
Bright Microlaser Microchip SB1 Laser SB1-1064 Pulsed DPSS Lasers, Microchip Lasers 0.008, 0.012, 0.040, 0.110, 0.150, 0.200, 0.225, 0.400 1064 2.0, 15.0, 20.0, 30.0, 40.0, 60.0, 80.0 400ps, 1.3ns 100Hz, 200Hz, 1kHz, 5kHz, 10kHz, 15kHz, 55kHz, 100kHz
Bright Microlaser Microchip SB1 Laser SB1-266 Pulsed DPSS Lasers, Microchip Lasers 0.002, 0.005 266 0.500, 1.0, 2.0 1.3ns 1kHz, 5kHz, 10kHz
Bright Microlaser Microchip SB1 Laser SB1-355 Pulsed DPSS Lasers, Microchip Lasers 0.010 355 Narrow 1.0 1.3ns 10kHz
Bright Microlaser Microchip SB1 Laser SB1-532 Pulsed DPSS Lasers, Microchip Lasers 0.004, 0.006, 0.010, 0.017, 0.020, 0.030, 0.040, 0.075, 0.100 532 0.300, 7.0, 10.0, 15.0, 20.0, 30.0, 40.0 400ps, 1.3ns 10Hz, 100Hz, 200Hz, 1kHz, 5kHz, 10kHz, 15kHz, 55kHz, 100kHz
Bright Microlaser Microchip SB1 Laser SB1-946 Pulsed DPSS Lasers, Microchip Lasers 0.004, 0.060, 0.070, 0.105 946 7.0, 12.0, 35.0 2ns 100Hz, 5kHz, 10kHz
Sirius-532: Picosecond DPSS lasers Sirius GR Pulsed DPSS Lasers, Ultrafast Lasers 2.0 532 32.0 10ps Single shot to 1MHz
Sirius-532: Picosecond DPSS lasers Sirius IR Pulsed DPSS Lasers, Ultrafast Lasers 5.0 1064 60.0 10ps Single shot to 1MHz
Wedge-B-1064 Wedge-HB/XB 1064 Pulsed DPSS Lasers 4.0 1064 2000.0, 4000.0 1.5ns Single shot to 1kHz, Single shot to 2kHz
Wedge-B-532 Wedge-HB/XB 532 Pulsed DPSS Lasers 2.0 532 1000.0, 2000.0 1.5ns Single shot to 1kHz, Single shot to 2kHz
Wedge-F-1064 Wedge-HF/XF 1064 Pulsed DPSS Lasers 1.5, 4.0 1064 70.0, 180.0 400ps - 1.5ns, 400ps - 1.6ns, 700ps - 2.5ns 10kHz to 100kHz, 50kHz to 200kHz
Wedge-F-266 Wedge-HF/XF 266 Pulsed DPSS Lasers 0.050, 0.180 266 5.0, 15.0 700ps - 1500ps, 400ps - 700ps 10kHz to 50kHz
Wedge-HF-355: 355nm compact nanosecond laser Wedge-HF/XF 355 Pulsed DPSS Lasers 0.800 355 40.0 500ps - 1ns 10kHz to 50kHz
Wedge-F-532 Wedge-HF/XF 532 Pulsed DPSS Lasers 0.500, 1.5, 2.0, 4.0 532 30.0, 40.0, 80.0, 120.0 500ps - 2ns, 700ps - 2ns, 400ps - 1.5ns, 400ps - 1ns 10kHz to 100kHz, 50kHz to 200kHz, Single shot to 10kHz
Wedge-XB 1572 Pulsed DPSS Lasers 0.800 1572 8000.0 2.5ns 1kHz
Wedge-XB 3um Pulsed DPSS Lasers 0.200 3106 100.0 3ns Single shot to 2kHz
more more

FL CW/CW Modulated Series

FL CW/CW Modulated SeriesThe FL CW/CW Modulated Series offers a variety of standard and custom CW fiber laser options. Available in both OEM and Turnkey formats, our 1um, 1.5um and 2um fiber lasers are manufactured to Telcordia standards and can be modified to meet your applications requirements. With available powers up to 100W at 1um, 30W at 1.5um and 40W at 2um, our CW fiber lasers are suitable for a wide range of applications. Available options and configurations include narrow linewidth, single frequency outputs, C and L-band broadband sources, PM fiber options, power tunability and high-speed trig./mod. Customized configurations are available upon request. 

FL-P Series

1um 1.5um Pulsed Fiber LasersThe FL-P series offers a variety of standard and custom pulsed fiber laser options. Available in both OEM and Turnkey formats, our 1um and 1.5um fiber lasers are manufactured to Telcordia standards and can be modified to meet your applications requirements. With available average powers up to 10W at 1um and 1.5um, peak powers up to 25kW, pulse widths in the range of 200ps to 100ns, and available options such as pulse monitoring, internal and external triggering, TTL or LVDS input signals, extended operating temperature range, and a robust design, our pulse series of fiber lasers are suitable for a wide range of applications. Customized configurations are available upon request.

Alcor Series

Alcor

The Alcor series is the most advanced femtosecond fiber laser emitting at 920 nm or 1064 nm, designed explicitly for multiphoton microscopy, with the 920-1 & 920-2 providing the best performance on the market with ultrashort, clean pulses, all at a competitive price. Our lasers offer high pulse energy, short and clean pulses, and ultimate peak powers. With an ultra-compact, robust, and air-cooled design, our lasers are highly stable and reliable with low maintenance. Our XSight and FLeX fiber external modules allow for precise power control and gating and GDD precompensation. The Alcor series provides a complete and reliable solution for multi-photon microscopy, with seamless OEM integration. 

Altair Series

Altair: Femtosecond Laser

The ALTAIR series produces high average powers up to 20W and ultrashort femtosecond pulses of <150 fs (<250 fs for VERSA configuration) at a high repetition rate of 80 MHz (others available). Ideal for bioimaging/biophotonics applications such as multiphoton microscopy, the ALTAIR provides high stability and excellent beam quality due to its mode-locked fiber design. It offers a range of options such as GDD pre-compensation, custom wavelengths, harmonics, repetition rates, and more. With a simplified fiber design that requires little to no maintenance compared to DPSS and Ti:Sapphire counterparts, the ALTAIR is the preferred solution for both OEM and researchers in the microscopy and life sciences fields.  

Antares Series

Antares

The ANTARES is a series of high-energy picosecond lasers designed for ease-of-use and integration in biophotonics and nonlinear spectroscopy applications. With a narrow linewidth, high repetition rate up to 80 MHz, excellent beam quality, and average powers from 1W to 40W at 1030 nm or 1064 nm, ANTARES is ideal for high-speed nonlinear spectroscopy applications such as CARS and FRET microscopy. Additional options include fine-tuning PRF synchronization, ultra-narrow linewidth, second and third harmonic options, custom repetition rates, and more.

Diadem Series

DIADEM Series

The DIADEM series is a compact, versatile, air-cooled, high-energy, ultrafast femtosecond laser, operating at 1030 or 1064 nm for advanced micro-machining applications. Available with up to 40µJ of pulse energy, pulse widths down to ≈ 400 fs, an M² < 1.2, and single-shot up to 2MHz repetition rate, the DIADEM has many pulse control features such as adjustable pulse duration, selectable repetition rate from 40 MHz down to single-shot, or fine pulse energy tuning so that pulses can be emitted in various modes (on demand via external signal, burst of pulses with configurable pulse separation). A 1300 nm version is also available for deep brain imaging. 

Microchip Series

Bright Microlaser Microchip SB1 Laser

The Microchip series is a line of ultra-compact, passively q-switched, single longitudinal mode (SLM), narrow linewidth, DPSS lasers that offer exceptional performance in a compact form factor. The lasers feature pulse durations ranging from 400 ps to 2 ns, energy levels up to 80 µJ, and repetition rates up to 100 kHz. Available in wavelengths from the UV to the NIR, this series is designed for OEM integrators and researchers working with LIDAR, 3D scanning, LIBS, night vision, and more. The lasers offer both nanosecond and picosecond options and are interchangeable with the same form factor and electrical and software interfaces across wavelengths, making them a flexible and versatile solution. 

Sirius Series

Sirius-532: Picosecond DPSS lasers

The SIRIUS series is a very compact, high-energy hybrid ultrafast laser for advanced micro-machining applications and supercontinuum pumping, offering over 60µJ and > 5 W @ 1064 nm, with pulses below 10 ps from single shot to 1MHz and an M² of < 1.2. SIRIUS is a fully configurable laser offering various pulse generation modes: selectable pulse durations and repetition rates, burst, gating and fine energy control. This laser series is specifically designed to allow for ergonomic and user-friendly operation. Also available in green or UV wavelengths, SIRIUS is ideally suited for selective ablation, electronics, and photonic applications.

Wedge Series

Wedge-XF-1064: 1064nm Picosecond Laser

The Wedge series is a line of DPSS lasers designed specifically for OEM applications such as micro machining of hard and soft materials, specialty marking, glass and crystal engraving, LIDAR, LIBS, spectroscopy, and medical diagnostics. These diode–pumped lasers, based on proprietary fast Q-switching technology, are compact, sealed, and monolithic which makes them insensitive to vibrations and harsh environments. With high peak powers and relatively low energy and heat generation, they allow efficient ablation and non-linear interaction with most materials. The compact and lightweight package is a great benefit in LIDAR and other aerospace applications, while short pulses provide extremely precise time-of-flight measurements.