Molecular Manipulation & Analysis Lasers

Flow Cytometry Cell Substructures

What are Molecular Manipulation & Analysis Lasers?

Molecular manipulation & analysis lasers in the field of biophotonics are specialized lasers (typically CW) used for studying and manipulating individual cells in biological systems. These lasers provide precise control and targeted energy delivery to enable various cellular analysis and manipulation techniques, providing researchers with powerful tools to explore cellular processes, understand cellular functions, and investigate disease mechanisms. These lasers offer precise control, high resolution, and versatility, enabling advancements in areas such as cell biology, neuroscience, regenerative medicine, and drug discovery. Common applications within this group include confocal fluorescence microscopy, DNA sequencing, flow cytometry, optogenetics, and more.

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Molecular Manipulation & Analysis Applications

Confocal Fluorescence Microscopy Lasers: Confocal microscopy is an optical technique that allows for extremely high spatial resolution imaging by placing two conjunct pinholes in the optical path. Most of the visible wavelengths can be used in confocal fluorescence microscopy, but it’s essential that all confocal fluorescence microscopy lasers (typically CW, low noise, single-mode, visible) have a high quality TEM00 beam profile.

DNA Sequencing Lasers: DNA sequencing or genome sequencing is an extremely powerful tool that allows the identification of the four basic DNA molecules adenine (A), cytosine (C), guanine (G), and thymine (T) by tagging them with fluorescence reporter molecules. Like all fluorescence applications, most of the visible wavelengths can be used in DNA sequencing, but it’s essential that all DNA sequencing lasers (typically CW, low noise, single-mode, visible) have a high quality TEM00 beam profile.

Flow Cytometry Lasers: Flow cytometry is a laser-based technology employed in cell counting, cell sorting, biomarker detection, and protein engineering. This technology is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Flow cytometry lasers (typically CW, low noise, single-mode, visible or IR) provide a high-quality TEM00 beam profile, low noise, and high pointing stability and power stability.

Optogenetics Lasers: Optogenetics is a biological technique that uses light to control cells in living tissue, typically neurons. While very little optical power is needed, optogenetics lasers (typically ns ps or fs pulsed, visible) are still the preferred delivery method because of their ability to be focused and targeted to specific areas of the brain.  Additionally, several wavelengths in the near-infrared region allow optogenetics lasers to be transmitted through bone and tissue eliminating the need for opening the skull.

Two-Photon Excitation Microscopy: Often called two-photon microscopy (2-photon, multi-photon, TPE microscopy), this process creates fluorescence images of live cells utilizing ultrashort pulsed lasers (typically mode-locked, fs pulsed lasers with NIR wavelengths). Compared to the usual one-photon microscopy, this method offers many benefits, such as better signal-to-noise ratio for clearer images, reduced photobleaching and phototoxicity, and deeper penetration in dense tissues.

Recommended Laser Series

We recommend the following laser series options for Molecular Manipulation & Analysis applications. There can often be a ton of options and many variables. Contact us today for help finding the perfect laser for your specific application!

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Matchbox Series

R1Z4-Matchbox Laser

The Matchbox series offers excellent performance and reliability in the “World’s Smallest” ultra-compact, all-in-one, integrated laser head. They can operate on a 5V power supply while maintaining low noise operation. The monolithic design of the Matchbox Series laser includes thermally stabilized optics in a hermetically sealed housing, ensuring reliable and maintenance-free operation. This series is available in wavelengths from 405 nm thru 1064nm, with options for collimated beam or fiber-coupled output, and single-mode and multimode versions.

Halite 2 Series

The Halite 2 series is a compact, single-box, all-fiber femtosecond laser designed to meet the most demanding applications in neuroscience, biophotonics, microscopy, and engineering. With pulses as short as <250 fs (< 100 fs op.), average power over 2 W at 1030 nm, and the option of second harmonic at 515 nm, it is an irreplaceable tool in every lab that needs a reliable, turn-key, ultrafast light source. Thanks to its unique construction and SESAM-free technology, it is a cost-effective solution that provides high pulse energy (over 100 nJ), excellent beam quality, and easy integration.

HL Series

TG420

The HL Series offers versatile and high-quality laser diode products in a variety of wavelengths and high-power options, ideal for direct imaging, industrial, and bio/medical applications. These reliable, efficient, and compact diodes come in TO-Can packages, making them perfect for OEM integration. Additionally, they operate with TE mode oscillation and are RoHS compliant, ensuring safe use. Choose the HL Series for quality, reliability, and performance in laser diodes. 

Jasper Series

The Jasper series is a diverse set of high-performance femtosecond fiber lasers providing different solutions based on a standard technology. The series includes the high-power Jasper X0 (200 µJ pulse energy / 60 W average power), the compact Jasper Micro (5 µJ pulse energy / 7 W average power) in a space-saving design, and the Jasper Flex for microprocessing (30 µJ pulse energy). Each model provides a fast warm-up time, long-term stability, and hands-free operation. Whether you need high power, compactness, or specific processing capabilities, the Jasper series has a solution.

LaserBoxx Low Noise Series

The LaserBoxx Low Noise series of CW diode laser modules & DPSS lasers offer wavelengths from 375 to 1064nm, output powers up to 500 mW, and OEM & plug-&-play modules. These rugged, compact & highly customizable lasers also offer excellent beam quality & stability, modulation capabilities, ultra-low noise output & options for SM, MM, and PM fiber coupling. Our dedicated control software, USB and RS232 interfaces, and external controller with power display make integration, operation, and remote diagnostics a breeze.

LaserBoxx SLM Series

The LaserBoxx-SLM series of SLM CW DPSS & diode laser modules are available in wavelengths from 532-1064 nm, delivering ultra-narrow line widths with excellent temperature stability and low noise current. Embedded firmware locks the laser on the same mode at each startup. This customizable, compact laser module is available in turn-key or OEM versions, with GUI for remote operation & diagnostics via USB, RS232, or direct I/O interface, with modulation capabilities and adjustable power options.

LDX Series

The LDX Series of made-in-the-US, high-power, multimode laser diodes provide wavelengths from 400nm – 1900nm, a wide range of output powers and package types, as well as completely customized solutions. We have been offering these high-quality laser diodes for over 25 years. The performance, reliability, and lifetimes are time tested, and if for some reason there is an issue, we will make it right. 

LXCc Series

R2Z3-L4Cc Combiner

The LXCc series is a range of compact, highly customizable, and flexible all-in-one laser combiners that provide the widest variety of wavelength options, up to 7 different laser lines (up to 500 mW output power per line), direct modulation on every source, SLM capabilities, proven long-term stability, and many other advanced features. The turnkey or OEM versions allow a large choice of lasers from 375nm up to 1064nm. The extension module provides the ultimate level of flexibility with options for up to 4 optical fiber outputs, AOTF modulator, motorized ND filter, integrated fast switching output ports for FRAP, or adjustable split power for light-sheet microscopy. 

Q-TUNE-IR Series

Q-TUNE-IRThe Q-TUNE-IR series is the perfect high peak power, coherent DPSS light source for researchers with infrared spectroscopy applications. This series uses an Optical Parametric Oscillator (OPO) to produce a tunable wavelength of 1380–4500nm (<10 cm-1 linewidth), achieving > 15mJ of pulse energy at the peak of the tuning curve. The Q-TUNE-IR requires little maintenance, with all laser electronics integrated into the housing, including an air-cooling system, eliminating the need for chillers or large power supplies while providing a guaranteed > 2 G shot pump diode lifetime.

Q-TUNE Series

Q-TUNE-IR

The Q-TUNE series is a highly efficient, air-cooled, tunable wavelength laser, providing up to 8mJ, shorter than 5ns pulses at up to 100Hz, designed for temporally resolved spectroscopy, metrology, photo-acoustic imaging, and remote sensing applications. This laser uses an optical parametric oscillator (OPO) to produce a tunable wavelength range of 410-2300 nm with a linewidth narrower than 6 cm-1, which can extend to 210-410 nm with an optional second harmonic generator. Options for air purging for UV optics, spectrometer, and fiber-coupled OPO output are also available.

Q-SHIFT Series

Q-Shift SeriesThe Q-SHIFT series of Q-switched DPSS lasers is designed for researchers and application specialists working in micromachining, dermatology, LIDAR, time-resolved laser spectroscopy, and LIBS applications. With its built-in nonlinear wavelength conversion stage, this series can produce unconventional DPSS wavelengths: 1163, 1177, 1300, 1317, 1551 & 1571 nm. The optional harmonics generator can generate up to the 4th harmonic for each fundamental wavelength, providing even more versatility. This series can produce up to 40mJ/1W @ 1163nm with up to 100Hz repetition rate.

Q-Spark

Quantas-Q-SPARK-266: High energy, compact, nanosecond, DPSS laser

The Q-SPARK series is an air-cooled, diode-pumped, Q-switched laser designed for researchers and application specialists working with ablation, LIDAR, remote sensing, and LIBS applications. The laser produces sub-nanosecond or nanosecond pulses with peak power up to 20 MW and pulse energies up to 20 mJ, making it ideal for a wide range of applications. With a short pulse (<800 ps to <2 ns), compact air-cooled package, and innovative water-free laser crystal end-pumping technology, the Q-SPARK series delivers high-quality, low divergence, Gaussian-like laser beams.

RHAML Series

The RHAML series of diode laser modules deliver a uniform, stable output beam that crucual for machine vision applcations, especially in 3D vision systems. The compact, RHAML series is a reliable light source that incoroporates a fucusing lens that can be easily adjusted by the user. Additionally, the RHAML series is fully IP67 compliant, making it an ideal solution for use in harsh environmental conditions and production/industrial facilities.

RML Series

The RML series of laser diode modules are available with single mode fiber outputs with a set wavelength from 405nm – 2500nm. The compact RML series is available with up to 80mW of output power in a package measuring 15mm in diameter and 40mm in length (without connector) . As an option these modules are offered with a potentiometer for power adjustment, external TTL modulation up to 1MHz and analog modulation up to 100kHz.

RWLD Series

The RWLD series offers competitively priced laser diodes with high beam quality and a wide variety of products to support multiple applications. Available in a wide range of wavelengths, power levels, and packages. The RWLD wavelength options span from 405nm to 1650nm, with output powers in the range of 10 mW to 300 mW, packaged in a TO-18 package with photodiode. We also offer customized options, such as a customized wavelength, output power, or a special package to meet your specific needs.

RWLP Series

R3Z0-WSLP-905-050m-M-PD: 905nm Multimode Fiber Coupled Laser Diode

The RWLP series offers an affordable and versatile solution for your laser application needs with single-mode and multimode options and wavelengths from 405nm thru the IR region. With customizable options, our team can work with you to solve any challenges you may face. Rigorously tested for long-term reliability, the RWLP series ensures consistent performance and high beam quality. Perfect for integration, this series supports multiple applications including biological and analytical instrumentation. 

SOL Series

SOL-03-532: 532nm Nanosecond Laser

The SOL series is the most compact, air-cooled, Q-switched DPSS nanosecond laser available in the power range from 4W to 40W @ 1064nm. With its excellent beam quality, high peak power, wavelength options (10W and 650µJ at 532nm / 4W @ 355nm), and wide range of add-ons, this series is ideal for the most demanding industrial and scientific applications. With a rugged, compact, lightweight, and easy-to-use single-unit design, the SOL laser is easy to integrate into any system, providing superior operational flexibility and performance/cost ratio.

TG Series

R1Z5-TG420

The TG Series of laser diodes emit in the blue spectral range with non-standard wavelengths from 418 nm up to 466 nm, perfect for Life Science applications, with a typical output power of 50mW and an absolute maximum output power of 100mW. Assembled in a 5.6 mm (TO-56) packages the TG series is a suitable for a wide range of OEM applications that require blue/violet light, including ECDL with low AR coating option.

Wedge Series

Wedge-XF-1064: 1064nm Picosecond Laser

The Wedge series of DPSS lasers is designed for OEM applications such as micromachining, LIDAR, LIBS, and more. Based on proprietary fast Q-switching technology, this series is compact, sealed, and monolithic, making them insensitive to vibrations & harsh environments. High peak powers, relatively low energy and low heat generation allow efficient ablation and non-linear interaction. Available from 266-3106nm, up to 8 mJ pulse energy @ 1064, the compact and lightweight package benefits LIDAR and aerospace applications, while short pulses provide exact time-of-flight measurements.