Ultra-fast mode-locked lasers have enabled two-photon microscopy, also referred to as multiphoton microscopy, to revolutionize the biological imaging. This technique now allows users to create high-resolution fluorescence images of living cells, without damaging the cells in the process.
In this article, we will explore how two-photon microscopy has evolved over the years and the integral role that the mode-locked laser has played in its evolution. It will also discuss the impact of laser wavelength on two-photon absorption and the advantages of working at 920 nm. Lastly, this article will introduce the development of the Alcor laser series by Spark Lasers, which is the ideal excitation source for two-photon microscopy when working at 920 nm and 1040 nm.