Multiphoton Microscopy Lasers

What is Multiphoton Microscopy?

Multi-photon microscopy, also called multiphoton microscopy, 2-photon microscopy or two-photon microscopy, is a widely used technique in the biological sciences, where sum-frequency generation within the sample can result in second harmonic generation or third harmonic generation to excite fluorescence in the sample.  This process is widely used for creating fluorescence images of live cells, since the multiphoton microscopy lasers are typically in the near-infrared wavelengths region, therefore reducing the potential of photochemical damage to the cells.  Due to the non-linear nature of this process, multiphoton microscopy lasers must have extremely short pulses, and therefore mode-locked femtosecond lasers are typically used for this application.   Additionally, as in all microscopy applications, multiphoton microscopy lasers, must also be single spatial mode to get diffraction-limited performance out of the microscope objective.   On this page you will find a list of our mode-locked multiphoton microscopy lasers, these mode-locked fiber lasers are ideal for multiphoton microscopy because of their short pulse width and excellent beam quality.

See the article in Laser Focus World about the Alcor Laser

Multi-Photon / Two-Photon Microscopy-Based Applications

Multi-photon microscopy is utilized for numerous life science applications throughout the fields of physiology, neurobiology, embryology, and tissue engineering. Near-transparent tissues (e.g., skin cells) can be easily visualized in great detail thanks to this microscopy method. Noninvasive optical biopsy procedures benefit from two-photon microscopy’s high-speed imaging capabilities. Localized chemical reactions can be produced when working within cell biology. Two-photon microscopy is also beneficial for cancer research (characterizing skin cancer, revealing tumor cell arrest, tumor cell-platelet interaction, etc.), in the neurosciences (characterizing intact neural tissues), and in brain in-vivo imaging. In-vivo imaging of the brain benefits from this process because of the ability for deep imaging in scattering tissue. Two-photon microscopy is often used to image the live-firing of neurons in model organisms, such as fruit flies, mice, and zebrafish.