Multi-Photon Microscopy Lasers

Multi-photon microscopy, also known as 2-photon microscopy, is a widely used technique in the biological sciences, where sum-frequency generation within the sample can result in second harmonic generation or third harmonic generation to excite fluorescence in the sample.  This process is widely used for creating fluorescence images of live cells, since the multiphoton microscopy lasers are typically in the near-infrared wavelengths region, therefore reducing the potential of photochemical damage to the cells.  Due to the non-linear nature of this process, multiphoton microscopy lasers must have extremely short pulses, and therefore mode-locked femtosecond lasers are typically used for this application.   Additionally, as in all microscopy applications, multiphoton microscopy lasers, must also be single spatial mode to get diffraction-limited performance out of the microscope objective.   On this page you will find a list of our mode-locked multiphoton microscopy lasers, these mode-locked fiber lasers are ideal for multiphoton microscopy because of their short pulse width and excellent beam quality.

See the article in Laser Focus World about the Alcor Laser

RPMC has years of experience supporting Multi-Photon Microscopy applications, helping industry professionals select the right tool for the job.

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In conclusion, if you have any questions or would like some assistance please contact us here. Furthermore, you can email us at to talk to a knowledgeable Product Manager. Alternatively, use the filters on this page to assist in narrowing down the selection of multi-photon microscopy lasers for sale. Finally, head to our Knowledge Center with our Lasers 101 page and Blogs, Whitepapers, and FAQ pages for further, in-depth reading.