Fluorescence Lifetime Lasers

Fluorescence Lifetime Lasers

Fluorescence lifetime is a spectroscopy method used for measuring the time a molecule spends in the excited state, by calculating the time between when the laser is fired, and the fluorescence signal is detected as well as the rise and decay time of the signal.  Typical lifetimes are on the order of a few picoseconds to hundreds of nanoseconds depending on the molecule, therefore ultra-short pulsed in the picosecond or femtosecond range are required from fluorescence lifetime lasers. Fluorescence lifetime is an intrinsic property, which does not depend on molecular concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity.  The lifetime is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers.  This makes it an ideal method for differentiating between molecules in messy biological systems, for example, fluorescence lifetime lasers have been shown to distinguish between healthy and cancers brain tissue in real time.  On this page, you will find a list of all of the full range of fluorescence lifetime lasers we offer at RPMC, including picosecond pulsed visible lasers and femtosecond mode-locked lasers which can be used to induce two-photon fluorescence.

To see a full list of other applications RPMC lasers are used for please check out our applications page by clicking here.