Confocal Fluorescence Microscopy Lasers

Confocal Fluorescence Microscopy Lasers

Confocal microscopy is an optical technique that allows for extremely high spatial resolution imaging by placing two conjunct pinholes in the optical path.  This provides for z-axis resolution as fine as 1 micron, making it possible to measure 3D depth profiles of materials.  In confocal fluorescence microscopy,  a laser is focused through the first pinhole and used to excite the sample, and then the emitted light is collected and focused through the second pinhole to the detector.  Most of the visible wavelengths can be used in confocal fluorescence microscopy, but it’s essential that all confocal fluorescence microscopy lasers have a high quality TEM00 beam profile.  On this page, you will find a list of all of the full range of confocal fluorescence microscopy lasers we offer at RPMC, including our multi-laser combiner.

To see a full list of other applications RPMC lasers are used for please check out our applications page by clicking here.