Confocal Fluorescence Microscopy Lasers
Confocal microscopy is an optical technique that allows for extremely high spatial resolution imaging by placing two conjunct pinholes in the optical path. This provides for z-axis resolution as fine as 1 micron, making it possible to measure 3D depth profiles of materials. In confocal fluorescence microscopy, a laser is focused through the first pinhole and used to excite the sample, and then the emitted light is collected and focused through the second pinhole to the detector. Most of the visible wavelengths can be used in confocal fluorescence microscopy, but it’s essential that all confocal fluorescence microscopy lasers have a high quality TEM00 beam profile. On this page, you will find a list of all of the full range of lasers used for this application, including our multi-laser combiners.
Confocal Microscopy Laser Source Requirements:
• Wavelengths: Most popular are 405nm, 488nm, 532nm/561nm, and 638nm. Others are often used as well. Wavelength combiners are ideal to cover all wavelengths.
• Output power: 50 – 500mW with good stability
• Beam Requirements: TEM00 & low noise. Usually 1x PM fiber-coupled.
RPMC has years of experience supporting Confocal Fluorescence Microscopy applications, helping industry professionals select the right tool for the job.
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