Confocal microscopy is an optical technique that allows for extremely high spatial resolution imaging by placing two conjunct pinholes in the optical path. This provides for z-axis resolution as fine as 1 micron, making it possible to measure 3D depth profiles of materials. In confocal fluorescence microscopy, a laser is focused through the first pinhole and used to excite the sample, and then the emitted light is collected and focused through the second pinhole to the detector. Most of the visible wavelengths can be used in confocal fluorescence microscopy, but it’s essential that all confocal fluorescence microscopy lasers have a high quality TEM00 beam profile. On this page, you will find a list of all of the full range of lasers used for this application, including our multi-laser combiners.
Lasers and Confocal Fluorescence Microscopy
Confocal Microscopy Laser Source Requirements:
• Wavelengths: Most popular are 405nm, 488nm, 532nm/561nm, and 638nm. Others are often used as well. Wavelength combiners are ideal to cover all wavelengths.
Multi-Wavelength Combiners: A Critical Tool for Fluorescence Imaging
CW multi-wavelength combiners have proven to be a critical component in Life Science applications, reducing time, cost, and complexity, and fundamentally changing these applications, allowing for increased efficiency and ease of use. Given the immense degree of complexity and time involved with combining multiple laser beams into a confocal microscope, multi-wavelength combiner modules have flooded the market in recent years. These plug-n-play style modules allow for swappable laser modules and quick and easy coupling with a confocal microscope, saving hours in precision alignment efforts. Laser combiner modules also help minimize the complexity of selecting multiple different wavelength sources from various laser models and even from various manufacturers, likely with different integration requirements. With a huge selection of wavelength combiners on the market, picking the right solution for your needs can be a difficult task. We provide a highly flexible, customizable series of multi-wavelength combiners, allowing for up to 7 excitation wavelengths of your choice in one user-friendly, compact unit.
Multi-Wavelength Laser Sources for Multi-Color Fluorescence Microscopy
Otto Heimstaedt and Heinrich Lehmann, where the first to realize the possibility of fluorescence microscopy while experimenting with ultraviolet microscopes between 1911 and 1913. While their initial intention was to increase the spatial resolution of the microscope by using shorter wavelength light, they quickly realized that ultraviolet excitation was inducing autofluorescence in their samples . However, these first instruments suffered from one major drawback, the high energy ultraviolet light would cause photochemical damage to the sample, especially living cells. Fortunately, in 1914 Stanislav Von Prowazek showed for the first time that fluorophores could be functionalized and bound to living cells  allowing the excitation and emission wavelengths to be engineered independently of the sample’s natural properties. Therefore, fluorescent tags quickly became standard practice in fluorescence microscopy allowing the excitation energy required to induce fluorescence to be significantly reduced. In turn, this decreased photochemical degradation of the sample by moving from ultraviolet to visible excitation wavelengths, which was extremely advantageous for biological studies allowing for sample integrity to be maintained especially for live samples. As a result, fluorescence microscopy has become one of the most widely utilized techniques in biological sciences. Figure 2 below shows an example of a cell where the three fluorophores where functionalized to attached themselves to the actin, mitochondria, and the nucleus .
The early generations of fluorescence microscopes utilized filtered atomic emission lamps, but most modern fluorescence microscopes employ single-mode lasers as the excitation source of choice. Solid-state lasers are far more reliable than gas discharge lamps, and their TEM00 beam profile provides the ability to be collimated and focused through complex optical geometries and focused down to a diffraction limited spot. This is especially important for confocal microscopes where the excitation source must be focused down through a pinhole to improve the z-resolution of the system. Lasers provide excellent spectral selectivity for exciting fluorophores, but unlike gas emission lamps, where the filters can be swapped to produce different excitation lines if a user wants to utilize a wide range of fluorophores in the same set-up, multiple laser sources will be required.
CW Solid-State Laser Sources for the Life Sciences: An Introduction to Selecting a Laser Module for your Experiment or Instrument.
As lasers become more commonplace for system integrators and research labs, many times the engineer or researcher knows the key parameters needed, but has difficulties sorting out the less important parameters, which could have a significant impact on cost, the experiment, or overall performance of the system. We will present what the important considerations are when selecting the laser source and how to define the specifications to insure you get the right laser for your application. Each application, and even the setup, will require specific parameters, if you are unsure, we will define the basic specifications needed for your experiment or your instrument.
With over 25 years experience providing confocal microscopy lasers to researchers and OEM integrators working in various markets and applications, and 1000s of units fielded, we have the experience to ensure you get the right product for the application. Working with RPMC ensures you are getting trusted advice from our knowledgeable and technical staff on a wide range of laser products. RPMC and our manufacturers are willing and able to provide custom solutions for your unique application.
If you have any questions, or if you would like some assistance please Contact Us here. Furthermore, you can email us at email@example.com talk to a knowledgeable Product Manager.
Alternatively, use the filters on this page to assist in narrowing down the selection of confocal microscopy lasers for sale. Finally, head to our Knowledge Center with our Lasers 101 page and Blogs, Whitepapers, and FAQ pages for further, in-depth reading.
The Matchbox series offers excellent performance and reliability in the “World’s Smallest” ultra-compact, all-in-one, integrated laser head. They can operate on a 5V power supply while maintaining low noise operation. The monolithic design of the Matchbox Series laser includes thermally stabilized optics in a hermetically sealed housing, ensuring reliable and maintenance-free operation. This series is available in wavelengths from 405 nm thru 1064nm, with options for collimated beam or fiber-coupled output, and single-mode and multimode version.
The LGK series of HeNe laser modulesboast an excellent TEM00 beam, robust mechanical design, a long service life of up to 30,000 hours, and are available in 543nm, 594nm, and 632.8nm wavelengths, with output powers up to 20mW. Choose between standard and customized models, with options for single or multimode, random or linear polarization, Brewster window tubes, and fiber coupling. Designed for long life, low noise, and high stability, with many customization options, these HeNe lasers are perfect for applications including spectroscopy, interferometry, holography, medical, and more!
The HL Series offers versatile and high-quality laser diode products in a variety of wavelengths and high-power options,ideal for direct imaging, industrial, and bio/medical applications. These reliable, efficient, and compact diodes come in TO-Can packages, making them perfect for OEM integration. Additionally, they operate with TE mode oscillation and are RoHS compliant, ensuring safe use. Choose the HL Series for quality, reliability, and performance in laser diodes.
The LaserBoxx Low Noise series of CW diode laser modules, with a variety of wavelengths from 375 to 785nm and output powers up to 350mW,offers highly customizable laser solutions for OEM and plug & play modules. With advanced features such as excellent beam quality, stability, and modulation capabilities, our lasers provide ultra-low noise and a wide range of options for SM, MM, and PM fiber coupling. Our dedicated control software, USB and RS232 interfaces, and external controller with power display make integration, operation, and remote diagnostics a breeze. Additionally, our rugged and compact design and wide variety of standard wavelengths ensure that our lasers can meet your specific needs.
The LGR series of HeNe laser replacement tubesprovide unparalleled performance for a wide range of applications.have a robust mechanical design,excellentbeam quality, and a long service lifeof up to 30,000 hours. Standardand customized models are available in a large variety in the red, green, and yellow spectral ranges withoutput powers up to 20 mW. Options for single–modeormultimode, random or linear polarization, and Brewster windowtubes for educational and scientific purposes.
The LXCc series is a range of compact, highly customizable, and flexible all-in-one laser combiners that provide the widest variety of wavelength options, up to 7 different laser lines (up to 500 mW output power per line), direct modulation on every source, SLM capabilities, proven long-term stability, and many other advanced features. The turnkey or OEM versions allow a large choice of lasers from 375nm up to 1064nm. The extension module provides the ultimate level of flexibility with options for up to 4 optical fiber outputs, AOTF modulator, motorized ND filter, integrated fast switching output ports for FRAP, or adjustable split power for light-sheet microscopy.
The R series of wavelength stabilized single–mode and multimode laser diodes offer narrow linewidth output in wavelengths from 633nm thru 1064nm. This highly customizable series offers package options ranging from components as basic as a TO-56 or 14-pin BF packaged diodes, to OEM modules including electronics, to UL/CE and IEC certified turn-key systems. The R series is the perfect source for various markets, including chemical analysis, bio-medical, fiber laser, and scientific applications.