MPX

1. Most compact & portable plug & play turn-key widefield and multiphoton microscope in the market.
2. Designed from scratch for non-experts and untrained users. All-integrated and fully software-controlled approach requires no prior know-how about femtosecond lasers, optics alignment, laser safety or other do´s and don’ts of complex laser scanning microscopes.
3. Integrated, permanently aligned wavelength tunable (765nm – 935nm) ultra-low noise femtosecond laser AND 1030nm fixed wavelength low-noise femtosecond laser. Both lasers are temporally synchronized and spatially overlapped.
4. Industry-leading laser scanning beam diameter of >22mm at objective back aperture to overfill state-of-the-art high NA, low magnification, long working distance objective.
5. Extra-large 2” achromatically compensated non-descanned detection optics for maximum fluorescence collection efficiency, lowest photo-bleaching, and phototoxicity. Captures fluorescence photons from a +/- 12° cone. Up to 4 special selected low dark count rate GaAsP PMTs, SiPMs, HPDs or other single photon counting detectors can be mounted.
6. Built-in multimodality at its best: Experience the convenience and power of multimodal imaging in a flexible, upgradable, modular package. “Widefield guided multi-photon imaging”. Combines high performance wide field epifluorescence and multiphoton imaging in a single fully flexible 360°- frontend (scanhead). Integrated widefield epi-fluorescence imaging with software-controlled (motorized) modality & objective change allows correlative widefield and multi-photon imaging across scales. It makes multimodal imaging super easy, super-fast focus finder, fast overview of specimen, ROI finder, WSI capable, “point and shoot” imaging.
7. Effortless transition from upright to inverted imaging with the same hardware. Upright, inverted, and oblique angle imaging within minutes, 5-axis high-precision motion control without laser alignment and optics tweaking. Enables easy and precise alignment to any experimental setting, such as implanted GRIN lenses, large mouse cages or perpendicular scanning through cranial windows.
8. Rugged industrial design for a broad range of user groups, especially for high usage core facilities with diverse imaging needs. Industry-ready, built for 24/7 operation. Permanently aligned lasers (widefield & multi-photon) maximize system up-time, enabling long imaging sequences, time laps, large scale mosaics & 3D stacks with maintained performance and image quality.
9. Large Area/Volume under the objective allows large complex setups e.g., for electrophysiology or life support setups for plants or animals or fluidic experiments.
10. Open layout: whole workspace and system not encapsulated in a tightly confined housing, thus enabling freedom to install any experimental setting during system lifetime.
11. Built-in workspace illumination underneath the objective for easy specimen handling in darkness. No need to switch on the flashlight of your smart phone to look for the sample.
12. Motorized & software-controlled 3 position objective turret with M32x075 threading.
13. Full remote control. Easy-to-use ChromoGazer™ software allows software-controlled modality changes, objective turret rotation, 5-axis motion control, complete system control and monitoring, settings, automatic error reporting and multimodal imaging. Microscope (lasers, imaging, modalities, objectives, etc.) can be fully remote controlled.
14. Highly portable, lightweight, easy to transport, and conveniently movable to almost any location. Whole microscope is easy to move around as it comes on wheels, can be used by other user groups in their respective labs. The microscope moves to the experiment, not the experiment to the microscope.
15. Air-cooled, no special facility requirements or laboratory construction needed, and minimal installation space.
16. Fiber delivered, built-in dual wavelength tunable femtosecond laser, therefore lowers safety risk from free-space beam and ensures permanent alignment.
17. No costly service contract necessary; fiber laser components are proven the most reliable, environmentally economic, easily serviceable, and have a low failure rate.
18. Whole microscope, including femtosecond laser, is manufactured, delivered, and serviced from one single manufacturer. One number to call. No blame-gaming between laser company and microscope company when the system does not meet specifications. Reduces risk, turnaround, cost, and component-confusion.
19. Modular design allows expandability and upgradable options, e.g. 3P, FLIM, CARS, SRS, etc., so the microscope can be updated and grow with your research.
20. The MPX can be equipped with a high-power multi-channel solid-state widefield illumination light source with external trigger function, combined with a highperformance sCMOS camera for epifluorescence widefield imaging.
21. The 360-frontend can be upgraded to resonant-galvo-galvo scanning; piezo controlled objective z-stacking and optogenetics.
22. The MPX runs industry leading scan software “ScanImage” with more than 1100 citations in peer-reviewed publications.
23. The microscope is powered by a built-in high-end PC, comes with a mouse, keyboard, display, all necessary software packages pre-installed and is completely air-cooled.

1. Resonant-galvo-galvo*: 30 fps at 512 x 512 pixels (8 kHz resonant galvo CRS8K) @ full FOV | * up to 100 Hz @ line scan
2. Motion Control / Upright and Inverted:
   1. Microscope Body (Scanhead) Motion:
      1. Fully flexible 360° scanhead for inverted, upright or oblique angle imaging. Up to 5-axis system with tip-tilt function. Easily changeable to an inverted setup within minutes by the user.
   2. Piezo Objective Scanner
      1. Various piezo objective scanners can be integrated for fast z-scanning
3. Adaptive Optics: Adaptive optics transmissive wavefront modulator (Phaseform) for enhanced image quality and penetration depth with up to 7th radial order Zernikes, 63 actuators.
4. Neuroexplorer Bundle: The Neuroexplorer has been unveiled for deep tissue imaging. This device combines various imaging techniques to simplify functional and intravital imaging. It offers bundles tailored for awake and sleeping mouse experiments, including features like stable head fixation and wireless vital signs monitoring during in vivo imaging.
5. FLIM: Providing pixel, line, frame clocks and laser synchronization for FLIM imaging. Detectors can be upgraded to single-photon counting. Easy upgradeable to widefield FD-FLIM.
6. Optogenetics: Photostimulation via dual-path galvo-galvo beam steering for simultaneous imaging and stimulation.
7. THG & 3P Imaging: Fully custom-designed laser scanning optics, achromatically compensated from 680 nm to 1700 nm, covering the full range of Ti:Sapphire lasers, fiber lasers as well as OPO/OPA/OPCPA systems up to 1700 nm allowing THG and 3P imaging.
8. CARS & SRS: MPX scan optics are optimized for label-free coherent Raman imaging. The MPX can be configured for CARS/SRS microscopy by using Prospective’s built-in FSX-dual output laser, a built-in time delay & AOM as well as detectors in epi and/or transmission.

Neurons (GCaMP) and Astrocytes (TexasRed) in the cortex:

Label-Free Mouse Colon

Single Cell Imaging

Neuronal Tracking in Drosophila