MPC

The MPC is designed for users who already have a femtosecond laser in their lab or want to buy a femtosecond laser from a different vendor (for 3P imaging for example).

The MPC is built around the Olympus epifluorescence widefield OEM building-block catalogue, allowing customizing illumination, binoculars, cameras etc. It incorporates a high-performance multiphoton scan engine BUT does not include a built-in femtosecond laser.

The laser scan engine of the MPC is designed to cover all wavelengths from 680nm up to 1700nm, it is therefore perfectly suited for correlative widefield, 2P, SHG, THG & 3P imaging.

Time-domain FLIM can easily be integrated with one of our partnering companies. Wide-field (frequency-domain) FLIM can be integrated with our partnering company Excelitas.

All femtosecond lasers from various vendors can be integrated. As Prospective is a laser engineering driven company, installation and integration of other lasers, beam characterization, dispersion control and debugging at the customer site are one of the core competencies. Therefore, we can offer out-of-one-hand 3P imaging solutions and excellent after sales customer support for non-laser or photonics savvy users.

The MPC is compatible with all ultrafast lasers enabling coherent Raman imaging application, like Prospective Instruments FSX laser source or from other vendors.

Due to its novel design, the MPC platform can be converted from upright to inverted or oblique angle imaging, however the external laser needs to be aligned after each transformation.

1. Built-in multimodality: “Widefield guided multi-photon imaging”. Combines highperformance wide field epifluorescence and multiphoton imaging in a single frontend (scanhead). Integrated widefield epifluorescence imaging with software-controlled (motorized) modality & objective change allows correlative widefield and multi-photon imaging across scales. It makes multimodal imaging super-easy, super-fast focus finder, fast overview of specimen, ROI finder, WSI capable, “point and shoot” imaging.
2. Fully custom-designed laser scanning optics, achromatically compensated from 680nm to 1700nm, covering the full range of Ti: Sapphire lasers, fiber lasers as well as OPO/OPA/OPCPA systems up to 1700nm.
3. Optics optimized for 2P, SHG, THG, coherent Raman imaging & 3P multiphoton imaging.
4. Industry-leading laser scanning beam diameter of >22mm at objective back aperture to overfill state-of-the-art high NA, low magnification, long working distance objective.
5. Extra-large 2” achromatically compensated non-descanned detection optics for maximum fluorescence collection efficiency, lowest photo-bleaching, and phototoxicity. Captures fluorescence photons from a +/- 12° cone. Up to 4 special selected low dark count rate GaAsP PMTs, SiPMs, HPDs or other single photon counting detectors can be mounted.
6. Motorized & software-controlled 3 position objective turret with M32x075 threading.
7. Large Area/Volume under the objective allows large complex setups e.g., for electrophysiology or life support setups for plants or animals or fluidic experiments.
8. Open Design: whole workspace and system not encapsulated in a tightly confined housing, thus enabling freedom to install any experimental setting during system lifetime.
9. Built-in workspace illumination underneath the objective for easy specimen handling in darkness. No need to switch on the flashlight of your smart phone to look for the sample.
10. Modular design allows expandability and upgradable options, e.g. 3P, FLIM, CARS, SRS, etc., so the microscope can be updated and grow with your research.
11. Easy-to-use software ChromoGazer™ developed for modality changes, whole system (incl. lasers) control, settings, and automatic error reporting.
12. Ultra compact, air-cooled, fits in every room, no need for special facility fittings. However, external lasers may well do so.

1. Resonant-galvo-galvo*: 30 fps at 512 x 512 pixels (8 kHz resonant galvo CRS8K) @ full FOV | * up to 100 Hz @ line scan
2. Motion Control / Upright and Inverted:
   1. Microscope Body (Scanhead) Motion:
      1. Fully flexible 360° scanhead for inverted, upright or oblique angle imaging. Scanhead can be mounted on a fixed stand or XYZ-stage.
   2. Piezo Objective Scanner
      1. Various piezo objective scanners can be integrated for fast z-scanning
3. Adaptive Optics: Adaptive optics transmissive wavefront modulator (Phaseform) for enhanced image quality and penetration depth with up to 7th radial order Zernikes, 63 actuators.
4. Neuroexplorer Bundle: The Neuroexplorer has been unveiled for deep tissue imaging. This device combines various imaging techniques to simplify functional and intravital imaging. It offers bundles tailored for awake and sleeping mouse experiments, including features like stable head fixation and wireless vital signs monitoring during in vivo imaging.
5. FLIM: Providing pixel, line, frame clocks and laser synchronization for FLIM imaging. Detectors can be upgraded to single-photon counting. Easy upgradeable to widefield FD-FLIM.
6. Optogenetics: Photostimulation via dual-path galvo-galvo beam steering for simultaneous imaging and stimulation.
7. THG & 3P Imaging: Fully custom-designed laser scanning optics, achromatically compensated from 680 nm to 1700 nm, covering the full range of Ti:Sapphire lasers, fiber lasers as well as OPO/OPA/OPCPA systems up to 1700 nm allowing THG and 3P imaging.
8. CARS & SRS: MPC scan optics is optimized for label free coherent Raman imaging. The MPC can be easily modified for CARS/SRS microscopy by using free-space external Laser coupling to the microscope body, detectors in transmission and by providing workspace for additional components e.g. time-delay, mirrors and other optics and electronics.

Neurons (GCaMP) and Astrocytes (TexasRed) in the cortex:

Label-Free Mouse Colon

Single Cell Imaging

Neuronal Tracking in Drosophila