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A group of neuroscientists were imaging awake animals and large cleared organs, where conventional confocal systems suffered from shallow penetration, high photodamage, and inability to capture label-free structural signals in thick samples. The team integrated a turnkey femtosecond source enabling multimodal nonlinear excitation—including two-photon fluorescence, SHG, and THG—paired with tissue-clearing compatibility and wide working distance objectives for macro-to-micro versatility.
Laser Solution: Free-space femtosecond laser, tunable in the near-IR with ultrashort pulses, used for multiphoton and harmonic generation microscopy.
Outcome: Unlocked deep, non-destructive 3D/4D imaging with reduced phototoxicity, revealing functional and structural biomarkers across scales in live and fixed specimens.