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A team of bio researchers was pursuing label-free lipid imaging in living organisms and tissue sections, but single-wavelength systems failed to target specific vibrational bonds while synchronization drifts degraded coherent signal strength. Utilizing a synchronized dual-output femtosecond laser with fixed and tunable arms optimized for the CH-stretch region, complete with built-in delay compensation for plug-and-play CARS contrast in open-table microscopes.
Laser Solution: Free-space dual-wavelength femtosecond laser, one fixed and one tunable in the near-IR with temporal overlap, used for coherent anti-Stokes Raman scattering (CARS).
Outcome: Facilitated high-specificity, non-invasive visualization of lipids and molecular distributions, advancing metabolic and neurological studies in model organisms and human samples.