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Mode-Locked Fiber Lasers for Two-Photon Microscopy: Ultra-fast mode-locked lasers have enabled two-photon microscopy, also referred to as multiphoton microscopy, to revolutionize the biological imaging. This technique now allows users to create high-resolution fluorescence images of living cells, without damaging the cells in the process.
For many years the advantages of fluorescence tags outweighed the disadvantages of the sample preparation requirements. However, with the emergence of two-photon fluorescence microscopy in the 1990s, many of these same advantages are achievable without any sample pretreatment. As a result, two-photon microscopy, also referred to as multiphoton microscopy, has once again revolutionized the field of biological imaging allowing for the creation of high-resolution images of living cells. Even today, new uses are constantly being discovered.
In this article, we will explore how two-photon microscopy has evolved over the years and the integral role that the mode-locked laser has played in its evolution. It will also discuss the impact of laser wavelength on two-photon absorption and the advantages of working at 920 nm. Lastly, this article will introduce the development of the Alcor laser series by Spark Lasers, which is the ideal excitation source for two-photon microscopy when working at 920 nm and 1040 nm.
If you have any questions, or would like assistance in selecting a Mode-Locked Fiber Laser for Two-Photon Microscopy, or other application, click below or talk to a knowledgeable laser Product Manager today by calling us at 636.272.7227 !