Multiphoton excitation requires high peak power pulses. Previously, wavelength tunable Ti:Sapphire lasers dominated this area, leading to the development of standard methods using a conventional pulse regime with typically 100-150 fs pulse duration, 80 MHz repetition rate, and watt level average power with specific wavelengths such as 800 nm, 920 nm, and 1040-1080 nm. Recently, femtosecond pulsed fiber lasers have started becoming the optimal solution due to their low relatively low fluence, limiting damage to living samples. Other advantages provided by fs fiber lasers include a more attractive price point, very compact and robust format, high electrical efficiency, high reliability, and less maintenance of cost of ownership. If you would like more details on why fs fiber lasers are becoming the optimal choice for multi-photon excitation applications, read this article: “Higher Power fs Fiber Lasers to Image Better, Deeper & Faster.” Get more information from our Lasers 101, Blogs, Whitepapers, FAQs, and Press Release pages in our Knowledge Center!
What is the best laser type for multi-photon microscopy?
What is the best laser type for multi-photon microscopy?