Multi-Photon Microscopy Lasers

Multi-Photon Microscopy Lasers Overview

Multi-Photon Microscopy

Multi-photon microscopy, also known as 2-photon microscopy, is a widely used technique in the biological sciences, where sum-frequency generation within the sample can result in second harmonic generation or third harmonic generation to excite fluorescence in the sample.  This process is widely used for creating fluorescence images of live cells, since the multiphoton microscopy lasers are typically in the near-infrared wavelengths region, therefore reducing the potential of photochemical damage to the cells.  Due to the non-linear nature of this process, multiphoton microscopy lasers must have extremely short pulses, and therefore mode-locked femtosecond lasers are typically used for this application.   Additionally, as in all microscopy applications, multiphoton microscopy lasers, must also be single spatial mode to get diffraction-limited performance out of the microscope objective.   On this page you will find a list of our mode-locked multiphoton microscopy lasers, these mode-locked fiber lasers are ideal for multiphoton microscopy because of their short pulse width and excellent beam quality.

See the article in Laser Focus World about the Alcor Laser

RPMC has years of experience supporting Multi-Photon Microscopy applications, helping industry professionals select the right tool for the job.

Contact Us today to let one of our knowledgeable Product Managers assist you!


Wavelength Selection

Power Selection

Energy Selection

Pulse Width Selection

Rep Rate Selection



3 results found

Alcor Series

Alcor Laser Series by Spark

ALCOR is the most advanced femtosecond fiber laser emitting at 920 nm or 1064 nm specifically designed for multiphoton microscopy instrumentation and industrial OEM integration, working with 24/7 operations, in an ultra-compact, robust and air-cooled format. Featuring the Alcor 920 4W, with the highest average power commercially available, as well as the XSight and FLeXSight external modules, allowing for fine and fast gating and power control, and GDD precompensation.

Altair Series

Altair Lasers Series by Spark

The ALTAIR is an ideal solution for biophotonics applications such as multiphoton microscopy where deep excitation of red-shifted indicators such as RCaMP, dtTomato, and MCherry is required.  Providing <160fs pulses, average power up to 20W and peak power up to 1.5MW at 1µm, the ALTAIR is the preferred solution for both OEM and researchers in the microscopy and lifesciences fields.  The fiber-based design ensures robust and reliable 24/7 operation while providing supreme pulse quality from a user friendly, ultra compact air-cooled package.