Multi Photon Microscopy Lasers

Multi Photon Microscopy Lasers

Multiphoton microscopy, also known as 2 photon microscopy, is a widely used technique in the biological sciences, where sum frequency generation within the sample can result in second harmonic generation or third harmonic generation to excite fluorescence in the sample.  This process is widely used for creating fluorescence images of live cells, since the multiphoton microscopy lasers are typically in the near infrared wavelengths region, therefore reducing the potential of photochemical damage to the cells.  Due to the non-linear nature of this process, multiphoton microscopy lasers must have extremely short pulses, and therefore mode-locked femtosecond lasers are typically used for this application.   Additionally, as in all microscopy applications, multiphoton microscopy lasers, must also be single spatial mode to get diffraction limited performance out of the microscope objective.   On this page you will find a list of our mode-locked multiphoton microscopy lasers, these mode-locked fiber lasers are ideal for multiphoton microscopy because of their short pulse width and excellent beam quality.

To see a full list of other applications RPMC lasers are used for please check out our applications page by clicking here.