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CartFluorescence Lifetime Lasers Overview
Fluorescence lifetime is a 
spectroscopy method used for measuring the time a molecule spends in the excited state, by calculating the time between when the laser is fired, and the fluorescence signal is detected as well as the rise and decay time of the signal. Typical lifetimes are on the order of a few picoseconds to hundreds of nanoseconds depending on the molecule, therefore ultra-short pulsed in the picosecond or femtosecond range are required from fluorescence lifetime lasers. Fluorescence lifetime is an intrinsic property, which does not depend on molecular concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity. The lifetime is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers. This makes it an ideal method for differentiating between molecules in messy biological systems, for example, fluorescence lifetime lasers have been shown to distinguish between healthy and cancers brain tissue in real-time. On this page, you will find a list of all of the full range of fluorescence lifetime lasers we offer at RPMC, including picosecond pulsed visible lasers and femtosecond mode-locked lasers which can be used to induce two-photon fluorescence.
RPMC has years of experience supporting Fluorescence Lifetime applications, helping industry professionals select the right tool for the job.
Contact Us today to let one of our knowledgeable Product Managers assist you!
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[description] => Fluorescence Lifetime Lasers Overview
Fluorescence lifetime is a spectroscopy method used for measuring the time a molecule spends in the excited state, by calculating the time between when the laser is fired, and the fluorescence signal is detected as well as the rise and decay time of the signal. Typical lifetimes are on the order of a few picoseconds to hundreds of nanoseconds depending on the molecule, therefore ultra-short pulsed in the picosecond or femtosecond range are required from fluorescence lifetime lasers. Fluorescence lifetime is an intrinsic property, which does not depend on molecular concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity. The lifetime is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers. This makes it an ideal method for differentiating between molecules in messy biological systems, for example, fluorescence lifetime lasers have been shown to distinguish between healthy and cancers brain tissue in real-time. On this page, you will find a list of all of the full range of fluorescence lifetime lasers we offer at RPMC, including picosecond pulsed visible lasers and femtosecond mode-locked lasers which can be used to induce two-photon fluorescence.
[su_spacer size="30"]
RPMC has years of experience supporting Fluorescence Lifetime applications, helping industry professionals select the right tool for the job.
Contact Us today to let one of our knowledgeable Product Managers assist you!
[su_spacer size="30"]
[su_button url="https://www.rpmclasers.com/contact/" target="blank" style="flat" background="#ff6600" size="10" center="yes"]Talk to a Product Manager[/su_button]
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[post_content] => Overview of Altair High-power, high repetition rate Femtosecond Laser for Bioimaging
The ALTAIR mode-locked fiber laser produces high average powers up to 20W, ultrashort femtosecond pulses of <160 fs, at a high repetition rate of 80 MHz (others available) in an air-cooled, ultra-compact and robust package. The ALTAIR is an ideal solution for bioimaging/biophotonics applications such as multiphoton microscopy where deep excitation of red-shifted indicators such as RCaMP, dtTomato, and MCherry is required. As a mode-locked fiber laser, the ALTAIR provides high stability and excellent beam quality. A host of options can be added to the system such as GDD pre-compensation down to -30,000 fs^2 (lower options available), custom wavelengths, harmonics, repetition rates, and more.
The Altair Femtosecond lasers are ideally suited for multi-photon microscopy applications. The fiber-based design enables a more compact, robust, and reliable laser than its DPSS and Ti:Sapphire counterparts. Through this simplified fiber design, the fiber lasers from SPARK require little to no maintenance. Historically, researchers have been using Ti: Sapphire lasers, which utilize many more components and moving parts, including water cooling systems, requiring significantly more maintenance and ultimately leading to higher total cost of ownership. The combination of short pulse duration and high average power offers many benefits for bioimaging such as lower scattering and deeper penetration and is the preferred solution for both OEM and researchers in the microscopy and life sciences fields
To find out more click here to download our white paper on how mode-locked lasers are used in two-photon microscopy.
Specifications:
- Up to 20 W avg. power at 80 MHz (1.5 MW peak)
- Up to 250 nJ/pulse
- <160 fs pulses
- Pulse picking
- GDD precompensation from 0 down to -30,000 fs^2
- Remote control through TCP/IP
- Rugged design
- Maintenance free
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[post_excerpt] => Femtosecond DPSS lasers
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[post_content] => Overview of Antares Narrow Linewidth Picosecond Laser
Spark Lasers, experts in ultra-faster lasers, offer the ANTARES series of compact high-energy picosecond lasers for biophotonics and nonlinear spectroscopy applications. The ANTARES laser system offers a quasi-continuous wave, 10 picosecond laser pulse, with a high repetition rate up to 80 MHz and a narrow linewidth. This laser series offers average powers from 1W up to 40W at 1030 nm or 1064 nm, and remarkable beam quality with M² < 1.2. This laser series is designed for ease-of-use and features a user-friendly compact, ergonomic design to help facilitate integration. The short pulse width and narrow linewidth of the ANTARES make it ideally suited for high-speed nonlinear spectroscopy applications such as Coherent Anti-stokes Raman spectroscopy (CARS) and Fluorescence Resonance Energy Transfer (FRET) microscopy. Spark Lasers offers additional options to the ANTARES series, including fine-tuning PRF synchronization of +/- 1 MHz, ultra-narrow linewidth < 2.5 nm (down to 0.01 nm), harmonic options, custom repetition rates, and more. Picosecond lasers are also well-suited for multi-photon microscopy applications. Lasing at 1030nm, the ANTARES offers many benefits for bioimaging including lower scattering and deeper penetration. To find out more click here to download our white paper on how mode-locked lasers are used in two-photon microscopy.Antares Specifications:
- Up to 40W at 1030 nm and 1064 nm
- Power modulation through GUI or TTL, including gating
- < 10 ps pulses
- >500 nJ per pulse
- Pulse picking
- 80 MHz Repetition Rate (40 MHz and 20 MHz optional)
- M² < 1.2
- Narrow Linewidth
- Rugged design
- Low total cost of ownership
Antares Applications:
- OPO Pumping
- Non-linear Optics
- Time-Correlated Single Photon Counting
- Semicon Inspection
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[post_content] => CPFL-1.5a – Pulsed Fiber Laser
The CPFL-1.5a: 1.5µm OEM Fiber Laser is a high-energy, pulsed, 1550nm, wavelength stabilized, OEM fiber laser from Bktel. This ultra-compact, q-switched series of lasers are designed for a wide range of applications that require high pulse repetition rates up to several megahertz and large average power. The CPFL-1.5a delivers up to 80µJ of pulse energy and a pulse width between 0.4 and 50 ns. High-speed triggering is available by external triggering, TTL, or LVDS make this laser ideal for integration into a wide range of high-speed applications. CPFL-1.5a: 1.5µm OEM Fiber Laser produces a perfect TEM00 output beam which can be delivered via an FC/APC fiber connector or an optional collimating lens. This laser requires less than 1 W pf power consumption and comes standard with RS-232 communication interface All of these features make the CPFL-1.5at laser series a robust tool for applications like LIDAR, 3D Scanning, and many others. [post_title] => CPFL-1.5a: 1.5µm OEM Fiber Laser [post_excerpt] => 1.5um CW OEM Fiber Laser [post_status] => publish [comment_status] => open [ping_status] => closed [post_password] => [post_name] => cpfl-1-5a-1-5%c2%b5m-oem-fiber-laser [to_ping] => [pinged] => [post_modified] => 2021-03-10 18:47:19 [post_modified_gmt] => 2021-03-10 18:47:19 [post_content_filtered] => [post_parent] => 0 [guid] => https://www.rpmclasers.com/?post_type=product&p=21405 [menu_order] => 0 [post_type] => product [post_mime_type] => [comment_count] => 0 [filter] => raw ) [3] => WP_Post Object ( [ID] => 21409 [post_author] => 5 [post_date] => 2018-03-16 20:47:00 [post_date_gmt] => 2018-03-16 20:47:33 [post_content] =>CPFL-1.5at – Pulsed Fiber Laser
The CPFL-1.5at: 1.5µm Stabilized Fiber Laser is a high energy, pulsed, 1550nm wavelength, stabilized, OEM fiber laser from Bktel. This ultra-compact, pulsed series of lasers are designed for a wide range of applications that require high pulse repetition rates up to several megahertz and large average power. The CPFL-1.5at delivers up to 80µJ of pulse energy and a pulse width between 0.2 and 5 ns. High-speed triggering is available by external triggering, TTL, or LVDS make this laser ideal for integration into a wide range of high-speed applications. CPFL-1.5at: 1.5µm Stabilized Fiber Laser produces a perfect TEM00 output beam which can be delivered via an FC/APC fiber connector or an optional collimating lens. This laser requires less than 20 W of power consumption and comes standard with RS-232 communication interface All of these features make the CPFL-1.5at laser series a robust tool for applications like LIDAR, 3D Scanning, and many others. [post_title] => CPFL-1.5at: 1.5µm Wavelength Stabilized OEM Fiber Laser [post_excerpt] => 1.5um CW OEM Fiber Laser [post_status] => publish [comment_status] => open [ping_status] => closed [post_password] => [post_name] => cpfl-1-5at-1-5%c2%b5m-wavelength-stabilized-oem-fiber-laser [to_ping] => [pinged] => [post_modified] => 2020-12-28 15:34:08 [post_modified_gmt] => 2020-12-28 15:34:08 [post_content_filtered] => [post_parent] => 0 [guid] => https://www.rpmclasers.com/?post_type=product&p=21409 [menu_order] => 0 [post_type] => product [post_mime_type] => [comment_count] => 0 [filter] => raw ) [4] => WP_Post Object ( [ID] => 21408 [post_author] => 5 [post_date] => 2018-03-16 20:44:00 [post_date_gmt] => 2018-03-16 20:44:58 [post_content] =>CPFL-1.5t – Pulsed Fiber Laser
The CPFL-1.5t: 1.5µm Ultra-Compact OEM Fiber Laser is a high-energy, pulsed, 1550nm, wavelength stabilized, OEM fiber laser from Bktel. This ultra-compact, pulsed series of lasers are designed for a wide range of applications that require high pulse repetition rates up to several megahertz and large average power. The CPFL-1.5t delivers up to 5 uJ of pulse energy and a pulse width between 0.4 and 50 ns. High-speed triggering is available by external triggering, TTL, or LVDS make this laser ideal for integration into a wide range of high-speed applications. CPFL-1.5t: 1.5µm Ultra-Compact OEM Fiber Laser produces a perfect TEM00 output beam which can be delivered via an FC/APC fiber connector or an optional collimating lens. This laser requires less than 150 mW pf power consumption and comes standard with RS-232 communication interface All of these features make the CPFL-1.5t laser series a robust tool for applications like LIDAR, 3D Scanning, and many others. [post_title] => CPFL-1.5t: 1.5µm Ultra-Compact OEM Fiber Laser [post_excerpt] => 1.5um CW OEM Fiber Laser [post_status] => publish [comment_status] => open [ping_status] => closed [post_password] => [post_name] => cpfl-1-5t-1-5%c2%b5m-ultra-compact-oem-fiber-laser [to_ping] => [pinged] => [post_modified] => 2020-12-28 15:34:09 [post_modified_gmt] => 2020-12-28 15:34:09 [post_content_filtered] => [post_parent] => 0 [guid] => https://www.rpmclasers.com/?post_type=product&p=21408 [menu_order] => 0 [post_type] => product [post_mime_type] => [comment_count] => 0 [filter] => raw ) [5] => WP_Post Object ( [ID] => 22580 [post_author] => 7 [post_date] => 2019-04-11 15:36:32 [post_date_gmt] => 2019-04-11 15:36:32 [post_content] =>CPFL2 – Pulsed Fiber Laser
The CPFL2: 1.5µm OEM Fiber Laser is a high-energy, pulsed, 1550nm wavelength, stabilized OEM fiber laser from Bktel. This ultra-compact, q-switched series of lasers is designed for a wide range of applications that require high pulse repetition rates up to several megahertz and large average power. The CPFL2 laser delivers up to 25µJ of pulse energy and a pulse width between 0.2 and 5 ns. High-speed triggering is available by external triggering, TTL, or LVDS, making this laser ideal for integration into a wide range of high-speed applications. The CPFL2: 1.5µm OEM Fiber Laser produces a perfect TEM00 output beam which can be delivered via an FC/APC fiber connector or an optional collimating lens. This laser requires less than 20 W pf power consumption and comes standard with RS-232 communication interface All of these features make the CPFL2 laser series a robust tool for applications like LIDAR, 3D Scanning, and many others. [post_title] => CPFL2: 1.5µm OEM Fiber Laser [post_excerpt] => 1.5um Pulsed OEM Fiber Laser [post_status] => publish [comment_status] => open [ping_status] => closed [post_password] => [post_name] => cpfl2-1-5%c2%b5m-oem-fiber-laser [to_ping] => [pinged] => [post_modified] => 2020-12-28 15:34:09 [post_modified_gmt] => 2020-12-28 15:34:09 [post_content_filtered] => [post_parent] => 0 [guid] => https://www.rpmclasers.com/?post_type=product&p=22580 [menu_order] => 0 [post_type] => product [post_mime_type] => [comment_count] => 0 [filter] => raw ) [6] => WP_Post Object ( [ID] => 18602 [post_author] => 5 [post_date] => 2017-04-24 18:25:00 [post_date_gmt] => 2017-04-24 18:25:17 [post_content] =>Overview of Diadem IR-10: High-power Femtosecond Laser
Spark Lasers, experts in mode-locked fiber lasers, offer the DIADEM IR series of compact femtosecond (fs) lasers for optogenetics and micromachining applications. DIADEM is a very compact, high energy ultrafast laser for advanced micromachining applications needing short femtosecond laser pulse widths. The DIADEM-IR10 offers 10µJ up to 1MHz, with pulses below 400fs and rep rate capability from single shot to 2 MHz with remarkable beam quality with M² < 1.2. The DIADEM laser series combines the most advanced electronics for on-the-fly pulse control with a compact, user-friendly design that requires less than 5 minutes of set-up time. The DIADEM IR has been specifically designed for demanding applications such as implantable medical device manufacturing, luxury watch manufacturing, consumers' electronics and optogenetics applications for instrumentation and industrial 24/7 OEM working operations. Harvard Medical School uses the DIADEM-IR mode-locked laser from Spark Lasers to facilitate cutting edge optogenetics research. To learn more about the use of these lasers for biophotonics click here.Diadem IR-10 features:
- 1030nm
- 10W average power
- 10uJ pulse energy
- 350fs – 10ps adjustable pulse duration
- Single shot to 2MHz
- M2 < 1.2
- Air-cooled
Diadem IR-10 applications:
- Optogenetics
- MicroMachining
Options:
- 1064nm option
- SHG, THG harmonics
- Ultra Sync: low timing jitter for ultra accurate, on-demand pulse triggering
- Extended PRF (up to 20MHz)
- Fiber coupling
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[post_excerpt] => Femtosecond DPSS lasers
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[post_content] => Overview of Diadem IR-20: High-power Femtosecond Laser
Spark Lasers, experts in mode-locked fiber lasers, offer the DIADEM IR series of compact femtosecond (fs) lasers for optogenetics and micromachining applications. DIADEM is a very compact, high energy ultrafast laser for advanced micromachining applications needing short femtosecond laser pulse widths. The DIADEM-IR20 offers 30µJ up to 700kHz, with pulses below 400 fs and rep rate capability from single shot to 2 MHz with remarkable beam quality with M² < 1.2. The DIADEM laser series combines the most advanced electronics for on-the-fly pulse control with a compact, user-friendly design that requires less than 5 minutes of set-up time. The DIADEM IR has been specifically designed for demanding applications such as implantable medical device manufacturing, luxury watch manufacturing, consumers' electronics and optogenetics applications for instrumentation and industrial 24/7 OEM working operations. Harvard Medical School uses the DIADEM-IR mode-locked laser from Spark Lasers to facilitate cutting edge optogenetics research. To learn more about the use of these lasers for biophotonics click here.Diadem IR-20 features:
- 1030nm
- 20W average power
- 30uJ pulse energy
- 350fs – 10ps adjustable pulse duration
- Single shot to 2MHz
- M2 < 1.2
- Air-cooled
Diadem IR-20 applications:
- Optogenetics
- MicroMachining
Options:
- 1064nm option
- SHG, THG harmonics
- Ultra Sync: low timing jitter for ultra accurate, on-demand pulse triggering
- Extended PRF (up to 20MHz)
- Fiber coupling
[post_title] => Diadem IR-20: Femtosecond Laser
[post_excerpt] => Femtosecond DPSS lasers
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[post_content] => Overview of Diadem IR-30: High-power Femtosecond Laser
Spark Lasers, experts in mode-locked fiber lasers, offer the DIADEM IR series of compact femtosecond (fs) lasers for optogenetics and micromachining applications. DIADEM is a very compact, high energy ultrafast laser for advanced micromachining applications needing short femtosecond laser pulse widths. The DIADEM-IR30 offers 40µJ of pulse energy up to 750kHz, with pulses below 400fs and rep rate capability from single shot to 2 MHz with a remarkable beam quality of M² < 1.2. The DIADEM laser series combines the most advanced electronics for on-the-fly pulse control with a compact, user-friendly design that requires less than 5 minutes of set-up time. The DIADEM IR has been specifically designed for demanding applications such as implantable medical device manufacturing, luxury watch manufacturing, consumers' electronics and optogenetics applications for instrumentation and industrial 24/7 OEM working operations. Harvard Medical School uses the DIADEM-IR mode-locked laser from Spark Lasers to facilitate cutting edge optogenetics research. To learn more about the use of these lasers for biophotonics click here.Diadem IR-20 features:
- 1030nm
- 30W average power
- 30uJ pulse energy
- 350fs – 10ps adjustable pulse duration
- Single shot to 2MHz
- M2 < 1.2
- Air-cooled
Diadem IR-20 applications:
- Optogenetics
- MicroMachining
Options:
- 1064nm option
- SHG, THG harmonics
- Ultra Sync: low timing jitter for ultra accurate, on-demand pulse triggering
- Extended PRF (up to 20MHz)
- Fiber coupling
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[post_excerpt] => Femtosecond DPSS lasers
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[post_date] => 2017-06-09 10:06:00
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[post_content] => GBS-C–Continuous Wave Fiber Laser
The GBS-C is a 1550nm high power continuous wave (CW) fiber laser from Bktel. These high brightness lasers provide up to 100 mW of output power from a single-mode fiber optic making them ideal for a wide range of industrial and scientific applications. The GBS-C offers output power tunability ranging from 10% to 100% of maximum output power and is available with either standard or polarization maintaining (PM) fiber depending on whether your application calls for linear or random polarization. The GBS-C laser produces a perfect TEM00 output beam which can be delivered via an FC/APC fiber connector or an optional collimating lens. High-speed triggering and modulation are available with modulation frequencies up to 8 kHz for of high-speed applications. This laser also comes standard with an RS-232/I2C via the USB communication interface. All of these features make the GBS-C laser series a robust tool for applications like welding, cladding, metrology, and many others.For more information please contact info@rpmclasers.com
[post_title] => GBS-C: Broadband Light Source [post_excerpt] => GBS-C Broadband Light Source [post_status] => publish [comment_status] => open [ping_status] => closed [post_password] => [post_name] => gbs-c [to_ping] => [pinged] => [post_modified] => 2020-12-28 15:35:05 [post_modified_gmt] => 2020-12-28 15:35:05 [post_content_filtered] => [post_parent] => 0 [guid] => https://www.rpmclasers.com/product/gfl/ [menu_order] => 0 [post_type] => product [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 10 [current_post] => -1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 16647 [post_author] => 5 [post_date] => 2017-04-03 21:06:00 [post_date_gmt] => 2017-04-03 21:06:00 [post_content] =>Overview of Altair High-power, high repetition rate Femtosecond Laser for Bioimaging
The ALTAIR mode-locked fiber laser produces high average powers up to 20W, ultrashort femtosecond pulses of <160 fs, at a high repetition rate of 80 MHz (others available) in an air-cooled, ultra-compact and robust package. The ALTAIR is an ideal solution for bioimaging/biophotonics applications such as multiphoton microscopy where deep excitation of red-shifted indicators such as RCaMP, dtTomato, and MCherry is required. As a mode-locked fiber laser, the ALTAIR provides high stability and excellent beam quality. A host of options can be added to the system such as GDD pre-compensation down to -30,000 fs^2 (lower options available), custom wavelengths, harmonics, repetition rates, and more. The Altair Femtosecond lasers are ideally suited for multi-photon microscopy applications. The fiber-based design enables a more compact, robust, and reliable laser than its DPSS and Ti:Sapphire counterparts. Through this simplified fiber design, the fiber lasers from SPARK require little to no maintenance. Historically, researchers have been using Ti: Sapphire lasers, which utilize many more components and moving parts, including water cooling systems, requiring significantly more maintenance and ultimately leading to higher total cost of ownership. The combination of short pulse duration and high average power offers many benefits for bioimaging such as lower scattering and deeper penetration and is the preferred solution for both OEM and researchers in the microscopy and life sciences fields To find out more click here to download our white paper on how mode-locked lasers are used in two-photon microscopy.Specifications:
- Up to 20 W avg. power at 80 MHz (1.5 MW peak)
- Up to 250 nJ/pulse
- <160 fs pulses
- Pulse picking
- GDD precompensation from 0 down to -30,000 fs^2
- Remote control through TCP/IP
- Rugged design
- Maintenance free
[post_title] => Altair: Femtosecond Laser
[post_excerpt] => Femtosecond DPSS lasers
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[post_modified] => 2021-03-30 18:34:18
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49 results found
