Flow Cytometry Lasers
What is Flow Cytometry?
Flow cytometry is a laser-based technology employed in cell counting, cell sorting, biomarker detection, and protein engineering. Most commonly utilized in the Life Sciences, this application is now also used in microbiology, food quality control, plant and animal cytology, immunology, and other areas. This technology is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. The fluorescence-labeled cell components are excited by the lasers to emit light at varying wavelengths. The fluorescence can then be measured to determine the amount and type of cells present in a sample. Up to thousands of particles per second can be analyzed as they pass through the liquid stream.
Flow Cytometry Based Applications
There are many different flow cytometry based applications. With multiple wavelengths at your disposal, allowing such diverse combinations as mentioned above, all of these applications are made more accessible. Some of these applications include cell sorting, immune cell phenotyping (immunophenotyping), immune cell function analysis, intracellular cytokine staining analysis, receptor occupancy analysis, gene therapy, cell cycle analysis, cell proliferation, membrane potential, live/dead bacteria discrimination, tumor suppressor gene/protein expression, antigen-specific cell responses, and many others.